Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Deininger, C; Wagner, A; Heimel, P; Salzer, E; Vila, XM; Weißenbacher, N; Grillari, J; Redl, H; Wichlas, F; Freude, T; Tempfer, H; Teuschl-Woller, AH; Traweger, A.
Enhanced BMP-2-Mediated Bone Repair Using an Anisotropic Silk Fibroin Scaffold Coated with Bone-like Apatite.
Int J Mol Sci. 2022; 23(1):
Doi: 10.3390/ijms23010283
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- Leading authors Med Uni Graz
- Deininger Christian
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- Abstract:
- The repair of large bone defects remains challenging and often requires graft material due to limited availability of autologous bone. In clinical settings, collagen sponges loaded with excessive amounts of bone morphogenetic protein 2 (rhBMP-2) are occasionally used for the treatment of bone non-unions, increasing the risk of adverse events. Therefore, strategies to reduce rhBMP-2 dosage are desirable. Silk scaffolds show great promise due to their favorable biocompatibility and their utility for various biofabrication methods. For this study, we generated silk scaffolds with axially aligned pores, which were subsequently treated with 10× simulated body fluid (SBF) to generate an apatitic calcium phosphate coating. Using a rat femoral critical sized defect model (CSD) we evaluated if the resulting scaffold allows the reduction of BMP-2 dosage to promote efficient bone repair by providing appropriate guidance cues. Highly porous, anisotropic silk scaffolds were produced, demonstrating good cytocompatibility in vitro and treatment with 10× SBF resulted in efficient surface coating. In vivo, the coated silk scaffolds loaded with a low dose of rhBMP-2 demonstrated significantly improved bone regeneration when compared to the unmineralized scaffold. Overall, our findings show that this simple and cost-efficient technique yields scaffolds that enhance rhBMP-2 mediated bone healing.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine Triphosphate - metabolism
- Animals - administration & dosage
- Anisotropy - administration & dosage
- Apatites - pharmacology
- Biomimetic Materials - pharmacology
- Bone Morphogenetic Protein 2 - pharmacology
- Bone Regeneration - drug effects
- Bone and Bones - drug effects, physiology, ultrastructure
- Caspase 7 - metabolism
- Caspases - metabolism
- Femur - diagnostic imaging, drug effects
- Fibroins - pharmacology
- Freezing - administration & dosage
- Humans - administration & dosage
- Male - administration & dosage
- Rats, Sprague-Dawley - administration & dosage
- Recombinant Proteins - pharmacology
- Tissue Scaffolds - chemistry
- Transforming Growth Factor beta - pharmacology
- X-Ray Microtomography - administration & dosage
- Rats - administration & dosage
- Find related publications in this database (Keywords)
- silk scaffold
- pseudarthrosis
- nonunion
- critical sized defect
- bone regeneration