Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Aronis, C; Villa, M.
Analysis of Cellular Metabolism Using Flow Cytometry.
Methods Mol Biol. 2026; 2990:119-125
Doi: 10.1007/978-1-0716-4997-8_10
PubMed
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- Führende Autor*innen der Med Uni Graz
- Aronis Christos
- Villa Matteo
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- Abstract:
- The understanding that cellular metabolism underlines the differentiation, activation, and function of immune cells has opened new avenues to modulate immunity. Here, we describe a method to analyze cellular metabolism using the quintessential immunological technique: flow cytometry. We analyze single cell suspensions, by combining the staining of surface immune cell markers and nutrient transporters, with the staining using metabolic dyes, to readout surrogates of metabolic pathway utilization with cell subset resolution.
- Find related publications in this database (using NLM MeSH Indexing)
- Flow Cytometry - methods
- Humans - administration & dosage
- Single-Cell Analysis - methods
- Animals - administration & dosage
- Biomarkers - metabolism
- Staining and Labeling - methods
- Metabolic Networks and Pathways - administration & dosage