Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Xue, B; Li, C; Zhang, B; Zhao, T; Shen, Z; Qiu, Z; Jin, M; Wang, J; Li, J.
Quantitative reverse transcription PCR to determine the inactivation of Human Rotavirus by chlorine.
Int J Hyg Environ Health. 2017; 220(4): 719-725.
Doi: 10.1016/j.ijheh.2017.02.007
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Zhao Tianyu
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- Abstract:
- Human rotaviruses (HRVs) are the major cause of acute diarrhea in infants and young children. Here, a real-time reverse transcription polymerase chain reaction assay targeting the rotaviral VP4 gene (VP4-RT-qPCR) was established to evaluate the inactivation of HRV upon chlorine disinfection, based on a previous report that damage to the 1227-2354bp region of the VP4 gene was associated with eliminated HRV infectivity by chlorine. In this study, inactivation of HRV by 0.6mg/L free chlorine was assessed in phosphate buffered saline (PBS; pH 7.2), and tap and river water samples, using both TCID50 and RT-qPCR (VP2- and VP4-RT-qPCR) assays, respectively. Among the samples tested, the VP2-RT-qPCR method did not show significant inactivation after chlorine disinfection; however, the reduction in VP4-RT-qPCR signal was correlated with decreased HRV infectivity. Moreover, the higher sensitivity of the VP4-RT-qPCR assay allowed for assessment of chlorine HRV inactivation at longer exposure times compared with the conventional TCID50 assay. Collectively, these results indicated that the VP4-RT-qPCR assay is a rapid, sensitive, and reliable tool to detect infectious HRV following chlorine inactivation, and highlights the potential for further development of qPCR/RT-qPCR assays to provide information regarding viral infectivity from drinking water plants.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Capsid Proteins - genetics
- Cell Line - administration & dosage
- Chlorine - toxicity
- Disinfection - administration & dosage
- Macaca mulatta - administration & dosage
- Reverse Transcriptase Polymerase Chain Reaction - administration & dosage
- Rotavirus - drug effects, genetics
- Virus Inactivation - drug effects
- Find related publications in this database (Keywords)
- Human rotavirus
- Chlorine
- Inactivation
- Infectivity
- RT-qPCR