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Selected Publication:
Leis, HJ; Ozand, PT; al Odaib, A; Gleispach, H.
Quantitative measurement of N-acetyl-L-aspartic acid in urine by gas chromatography with negative-ion chemical ionization mass spectrometry.
J Chromatogr. 1992; 578(1):116-119
Doi: 10.1016/0378-4347(92)80232-F
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- Leading authors Med Uni Graz
- Leis Hans-Joerg
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- Abstract:
- A highly specific and sensitive assay for N-acetyl-L-aspartic acid has been developed. The trideuterated compound was synthesized and used as an internal standard for gas chromatography with negative-ion chemical ionization mass spectrometry. Urine samples were acidified and extracted with ethyl acetate, and the compounds converted into their pentafluorobenzyl ester derivatives. Under these conditions, sub-picogram amounts of the pure derivatives could be detected. Thus, only microliter volumes of urine samples have to be processed to achieve reliable quantification of "basal" levels of N-acetyl-L-aspartic acid.
- Find related publications in this database (using NLM MeSH Indexing)
- Aspartic Acid - analogs and derivatives
- Chromatography, Gas - analogs and derivatives
- Humans - analogs and derivatives
- Mass Spectrometry - methods