Medizinische Universität Graz - Research portal
Selected Publication:
Schmut, O; Faulborn, J; Trummer, G.
Quantifying the damage to conjunctival and corneal cell cultures caused by uv light using CASY (Cell Analysis System). A method for reducing animal experiments
Ophthalmologe. 1999; 96(6):375-381
Doi: 10.1007/s003470050421
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- Leading authors Med Uni Graz
- Schmut Otto
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- Abstract:
- BACKGROUND: By depletion of stratospheric ozone, enhanced levels of UV radiation reach the surface of the earth. Exposure of the anterior parts of the eye to UV radiation leads to irritation of the conjunctival and corneal cells. METHOD: By the CASY (cell analysis) system the influence of UV radiation on cultures of conjunctival and corneal cells was observed by determination of the cell counts, cell diameter, and the cell volume. RESULTS: By comparing these parameters with the control, damage of the conjunctival and corneal cells by UV radiation can be determined within 2 s of exposure of the cells in quartz glass vials to the UV light. CONCLUSION: By the CASY system the dramatic influence of UV radiation on cells of the anterior parts of the eye can be determined. This system enables objective statements on the cytotoxicity of radiation, chemical substances and drugs on cell cultures without the use of radioactive methods, complicated determination of cell metabolism or staining methods which are difficult to standardize. Also, studies on animals can be reduced by the CASY system.
- Find related publications in this database (using NLM MeSH Indexing)
- Animal Testing Alternatives -
- Animals -
- Cattle -
- Cell Count - radiation effects
- Cell Size - radiation effects
- Cells, Cultured - radiation effects
- Conjunctiva - drug effects
- Cornea - drug effects
- Dose-Response Relationship, Radiation - drug effects
- Image Processing, Computer-Assisted - instrumentation
- Radiation Injuries, Experimental - pathology
- Sheep - pathology
- Ultraviolet Rays - adverse effects
- Find related publications in this database (Keywords)
- UV Radiation
- Irritation
- Conjunctival and Corneal Cells
- Cell Analysis System