Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Sattler, W; Leis, HJ; Kostner, GM; Malle, E.
Quantification of 7-dehydrocholesterol in plasma and amniotic fluid by liquid chromatography/particle beam-mass spectrometry as a biochemical diagnostic marker for the Smith-Lemli-Opitz syndrome.
Rapid Commun Mass Spectrom. 1995; 9(13):1288-1292
Doi: 10.1002/rcm.1290091313
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- Führende Autor*innen der Med Uni Graz
- Sattler Wolfgang
- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
- Leis Hans-Joerg
- Malle Ernst
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- Abstract:
- The qualitative and quantitative determination of cholesterol and 7-dehydrocholesterol (7-DHC) in plasma as a biochemical diagnostic marker for the Smith-Lemli-Opitz syndrome by liquid chromatography/particle beam interface-mass spectrometry (LC/PB-MS) is presented. Baseline separation of cholesterol and 7-DHC is achieved on a silica column with hexane+ethanol (99: 1 v/v) as mobile phase within a 10 min analysis. Recoveries of cholesterol and 7-DHC in a simple two-phase extraction system are nearly 100%. The absolute limit of detection using LC/PB-MS is approximately 10 ng. The method presented allows extraction, analysis and quantification of cholesterol and 7-DHC within 15 min without the necessity of sample derivatization.
- Find related publications in this database (using NLM MeSH Indexing)
- Amniotic Fluid - chemistry
- Biological Markers - chemistry
- Cholesterol - blood
- Chromatography, Liquid - blood
- Dehydrocholesterols - analysis
- Female - analysis
- Humans - analysis
- Mass Spectrometry - analysis
- Pregnancy - analysis
- Smith-Lemli-Opitz Syndrome - blood