Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Berens, C; Heine, A; Müller, J; Held, SAE; Mayer, K; Brossart, P; Oldenburg, J; Pötzsch, B; Wolf, D; Rühli, H.
Variable resistance to freezing and thawing of CD34-positive stem cells and lymphocyte subpopulations in leukapheresis products
CYTOTHERAPY. 2016; 18(10): 1325-1331.
Doi: 10.1016/j.jcyt.2016.06.014
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Heine Annkristin
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- Abstract:
- Background aims. Leukapheresis products for hematopoietic stem cell transplantation can be cryopreserved for various indications. Although it is known that CD34(+) cells tolerate cryopreservation well, a significant loss of CD3(+) cells has been observed, which has been ascribed to several factors, including transport, storage conditions and granulocyte-colony stimulating factor (G-CSF) administration. Methods. To assess the tolerance of CD34(+) cells and lymphocyte subpopulations for cryopreservation and thawing, the post-thaw recoveries of CD34(+) cells, CD3(+)CD4(+) cells, CD3(+)CD8(+) cells, CD19(+) cells and CD16(+)CD56(+) cells were determined in 90 cryopreserved apheresis products, among which 65 were from G-CSF-mobilized donors, and 34 from unrelated donors that underwent transport before cryopreservation at our center. A controlled rate freezer and 5% dimethyl sulfoxide were used for cryopreservation. Results. We could detect statistically significant differences for CD34(+) cell recovery (93.0 +/- 20.7%) when compared to CD3(+)CD4(+) cell (83.1 +/- 15.4%, P= 0.014), and CD3(+)CD8(+) cell recovery (83.3 +/- 13.9%, P= 0.001). Similarly, CD19(+) cell recovery (98.6 +/- 15.1%) was higher than CD3(+)CD4(+) cell = 2.5 x 10(-7)) and CD3(+)CD8(+) cell recovery (P = 1.2 x 10(-8)). Post-thaw recovery rates of all cell populations were not impaired in G-CSF mobilized products compared with non-mobilized products nor in unrelated compared with related donor products. Discussion. Our data suggest a lower tolerance of CD3(+) cells for cryopreservation and demonstrate that freezing thawing resistance thawing is cell-specific and independent from other factors that affect post-thaw recovery of cryopreserved cells. Thus, a clinical consequence may be the monitoring of post-thaw CD3(+) cell doses of cryopreserved products, such as donor lymphocyte infusions.
- Find related publications in this database (Keywords)
- B cells
- cryopreservation
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- hematopoietic stem cell transplantation
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