Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kobetitsch, S; Gierlikowska, B; Kunert, O; Mazen, AMA; Raab, P; Kretschmer, N; Donolo, C; Pirker, T; Bauer, R; Kiss, AK; Pferschy-Wenzig, EM.
Salvadora persica leaves: phytochemical profile and in vitro-inhibitory activity on inflammatory mediators implicated in periodontal disease.
Pharm Biol. 2024; 62(1): 563-576.
Doi: 10.1080/13880209.2024.2374801
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- Co-Autor*innen der Med Uni Graz
- Kretschmer Nadine
- Pirker Teresa Marie
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- Abstract:
- CONTEXT: Virtually all parts of Salvadora persica L. (Salvadoraceae) are used in traditional medicine. The twigs and leaves are used for oral health, but leaves are far less investigated. OBJECTIVE: This study assesses the oral health-promoting potential of S. persica leaves with emphasis on anti-inflammatory and antiproliferative effects and provides an in depth-characterization of their metabolite profile. MATERIALS AND METHODS: Hot-water and methanolic S. persica leaf extracts (1, 10, and 100 µg/mL) and their major constituents (5, 10, and 50 µM), were subjected to cellular assays on IL-8 and TNFα release in LPS-stimulated human neutrophils, NO-release in LPS/IFNγ stimulated mouse macrophages, and proliferation of HNO97 human tongue carcinoma cells. Metabolite profiling was performed by UHPLC-HRMS analysis. Major constituents were isolated and structurally elucidated. RESULTS AND DISCUSSION: Both extracts showed pronounced anti-inflammatory activity in LPS-stimulated neutrophils. Major identified compound classes were flavonoid glycosides, the glucosinolate glucotropaeolin, phenyl- and benzylglycoside sulfates, and megastigmane glycosylsulfates, the latter ones identified for the first time in S. persica. Glucotropaeolin strongly inhibited the release of IL-8 and TNF-α (13.3 ± 2.0 and 22.7 ± 2.6% of the release of stimulated control cells at 50 µM), while some flavonoids and 3-(3'-O-sulfo-β-d-glucopyranosyloxy)-7,8-dihydro-β-ionone, a newly isolated megastigmane glycosylsulfate, were moderately active. Benzylisothiocyanate, which is likely formed from glucotropaeolin during traditional application of S. persica, showed considerable antiproliferative activity (IC50 in HNO97 cells: 10.19 ± 0.72 µM) besides strongly inhibiting IL-8 and TNFα release. CONCLUSIONS: Glucotropaeolin and benzylisothiocyanate are likely implicated in the oral health-promoting effects of S. persica leaves. The chemistry and pharmacology of the newly identified megastigmane glycosylsulfates should be further evaluated.
- Find related publications in this database (using NLM MeSH Indexing)
- Plant Leaves - administration & dosage
- Humans - administration & dosage
- Plant Extracts - pharmacology, isolation & purification
- Animals - administration & dosage
- Mice - administration & dosage
- Anti-Inflammatory Agents - pharmacology, isolation & purification
- Salvadoraceae - chemistry
- Inflammation Mediators - metabolism, antagonists & inhibitors
- Periodontal Diseases - drug therapy
- Neutrophils - drug effects, metabolism
- Cell Proliferation - drug effects
- Cell Line, Tumor - administration & dosage
- Tumor Necrosis Factor-alpha - metabolism
- Macrophages - drug effects, metabolism
- Dose-Response Relationship, Drug - administration & dosage
- RAW 264.7 Cells - administration & dosage
- Interleukin-8 - metabolism
- Phytochemicals - pharmacology, isolation & purification
- Find related publications in this database (Keywords)
- Toothbrush tree
- anti-inflammatory
- antiproliferative
- UHPLC-HRMS metabolite profiling
- megastigmane