Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Steiner, A; Favre, S; Mack, M; Hausharter, A; Pillet, B; Hafner, J; Mitterer, V; Kressler, D; Pertschy, B; Zierler, I.
Dissecting the Nuclear Import of the Ribosomal Protein Rps2 (uS5).
Biomolecules. 2023; 13(7):
Doi: 10.3390/biom13071127
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- Leading authors Med Uni Graz
- Steiner Andreas
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- Abstract:
- The ribosome is assembled in a complex process mainly taking place in the nucleus. Consequently, newly synthesized ribosomal proteins have to travel from the cytoplasm into the nucleus, where they are incorporated into nascent ribosomal subunits. In this study, we set out to investigate the mechanism mediating nuclear import of the small subunit ribosomal protein Rps2. We demonstrate that an internal region in Rps2, ranging from amino acids 76 to 145, is sufficient to target a 3xyEGFP reporter to the nucleus. The importin-β Pse1 interacts with this Rps2 region and is involved in its import, with Rps2 residues arginine 95, arginine 97, and lysine 99 being important determinants for both Pse1 binding and nuclear localization. Moreover, our data reveal a second import mechanism involving the N-terminal region of Rps2, which depends on the presence of basic residues within amino acids 10 to 28. This Rps2 segment overlaps with the binding site of the dedicated chaperone Tsr4; however, the nuclear import of Rps2 via the internal as well as the N-terminal nuclear-targeting element does not depend on Tsr4. Taken together, our study has unveiled hitherto undescribed nuclear import signals, showcasing the versatility of the mechanisms coordinating the nuclear import of ribosomal proteins.
- Find related publications in this database (using NLM MeSH Indexing)
- Ribosomal Proteins - metabolism
- Active Transport, Cell Nucleus - administration & dosage
- Cell Nucleus - metabolism
- Ribosomes - metabolism
- Arginine - metabolism
- Amino Acids - metabolism
- Protein Binding - administration & dosage
- Find related publications in this database (Keywords)
- ribosomal protein
- ribosome assembly
- nuclear import
- Rps2
- uS5
- importin
- Pse1
- Kap121
- dedicated chaperone
- yeast