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SHR Neuro Cancer Cardio Lipid Metab Microb

Bourgeois, B; Madl, T.
Methods to Study Structure and Dynamics of FOXO Proteins.
Methods Mol Biol. 2025; 2871: 85-98. Doi: 10.1007/978-1-0716-4217-7_8
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Leading authors Med Uni Graz: Bourgeois Benjamin Michel Rene; Madl Tobias
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Abstract:: FOXOs are highly dynamic transcription factors consisting of one conserved DNA-binding domain (forkhead domain) as well as intrinsically disordered regions (IDRs) at the N- and C-termini. These IDRs are essential and regulate transcriptional activity of FOXOs by serving as interaction platform for cofactors. Furthermore, the IDRs are involved in intra- and intermolecular homeotypic and heterotypic interactions between FOXOs and in turn mediate FOXO auto-inhibition and condensate formation. Here, we describe generalizable methods to study the structure and dynamics of FOXO proteins in vitro using a combination of biophysical techniques, in particular nuclear magnetic resonance spectroscopy. These methods can serve as a blueprint for the investigation of other IDR-containing transcription factors.
Find related publications in this database (using NLM MeSH Indexing): Forkhead Transcription Factors - metabolism, chemistry; Humans - administration & dosage; Intrinsically Disordered Proteins - chemistry, metabolism; Magnetic Resonance Spectroscopy - methods; Protein Conformation - administration & dosage; Protein Binding - administration & dosage; Nuclear Magnetic Resonance, Biomolecular - methods