Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Splith, K; Fellmer, P; Matia, I; Varga, M; Oliverius, M; Kuhn, S; Feldbrügge, L; Krenzien, F; Hau, HM; Wiltberger, G; Schmelzle, M; Jonas, S.
Antibody-mediated rejection of arterialised venous allografts is inhibited by immunosuppression in rats.
PLoS One. 2014; 9(3): e91212
Doi: 10.1371/journal.pone.0091212
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- Co-Autor*innen der Med Uni Graz
- Hau Hans-Michael
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- Abstract:
- OBJECTIVES AND DESIGN: We determined in a rat model (1) the presence and dynamics of alloantibodies recognizing MHC complexes on quiescent Brown-Norway (BN) splenic cells in the sera of Lewis (LEW) recipients of Brown-Norway iliolumbar vein grafts under tacrolimus immunosuppression; and (2) the presence of immunoglobulins in the wall of acute rejected vein allografts. MATERIALS AND METHODS: Flow cytometry was used for the analysis of day 0, 14 and 30 sera obtained from Lewis recipients of isogeneic iliolumbar vein grafts (group A) or Brown-Norway grafts (group B, C) for the presence of donor specific anti-MHC class I and II antibodies. Tacrolimus 0.2 mg/kg daily was administered from day 1 to day 30 (group C). Histology was performed on day 30. RESULTS: Sera obtained preoperatively and on day 30 were compared in all groups. The statistically significant decrease of anti MHC class I and II antibody binding was observed only in allogenic non-immunosuppressed group B (splenocytes: MHC class I - day 0 (93% ± 7% ) vs day 30 (66% ± 7%), p = 0.02, MHC class II - day 0 (105% ± 3% ) vs day 30 (83% ± 5%), p = 0.003; B-cells: MHC class I - day 0 (83% ± 5%) vs day 30 (55% ± 6%), p = 0.003, MHC class II - day 0 (101% ± 1%) vs day 30 (79% ± 6%), p = 0.006; T-cells: MHC class I - day 0 (71% ± 7%) vs day 30 (49% ± 5%), p = 0.04). No free clusters of immunoglobulin G deposition were detected in any experimental group. CONCLUSION: Arterialized venous allografts induce strong donor-specific anti-MHC class I and anti-MHC class II antibody production with subsequent immune-mediated destruction of these allografts with no evidence of immunoglobulin G deposition. Low-dose tacrolimus suppress the donor-specific antibody production.
- Find related publications in this database (using NLM MeSH Indexing)
- Allografts - immunology
- Animals - administration & dosage
- Antibody-Dependent Cell Cytotoxicity - drug effects, immunology
- Arteries - transplantation
- Graft Rejection - drug therapy, immunology
- Histocompatibility Antigens Class I - immunology, metabolism
- Histocompatibility Antigens Class II - immunology, metabolism
- Immunosuppression Therapy - administration & dosage
- Isoantibodies - immunology
- Lymphocyte Subsets - immunology, metabolism
- Male - administration & dosage
- Rats - administration & dosage
- Spleen - cytology, immunology, metabolism
- Time Factors - administration & dosage
- Transplantation Immunology - administration & dosage
- Veins - immunology, transplantation