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Selected Publication:
Desoye, G; Ertl-Stockinger, U; Porta, S.
Prolonged administration in vivo of alpha and beta adrenergic agonists decreases insulin binding to rat myocardial membranes in vitro by different mechanisms.
LIFE SCI 1991 48: 2249-2258.
Doi: 10.1016%2F0024-3205%2891%2990340-H
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- Leading authors Med Uni Graz
- Desoye Gernot
- Co-authors Med Uni Graz
- Porta Sepp
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- Abstract:
- Male Sprague Dawley rats were continuously treated in vivo for 6, 12 and 20 hours with a combination of an alpha- (beta-) adrenoreceptor agonist and a beta- (alpha-) adrenoreceptor antagonist in subcutaneously implanted depot tablets. Crude membranes prepared from myocardial cells exhibited a decreased maximum binding of [125I]-insulin after 20 hours irrespective of the treatment applied. Scatchard and non-linear regression analysis of the displacement curves assuming two non-cooperative binding sites revealed a downregulation of the high affinity receptors for about 85% and a concomitant 2.5-fold increased receptor affinity under beta-adrenergic influence. In contrast, alpha-adrenergic treatment did not affect the receptor number but decreased the high affinity by 70%. The low affinity binding sites were virtually unaffected by the different treatments. The phospholipid and cholesterol contents of the membranes were not significantly altered. The phospholipid/cholesterol ratios after 12 and 20 hours of alpha-adrenergic treatment, however, were decreased. We suggest that the decreased binding activity of insulin receptors on rat myocardial membranes after continuous in vivo treatment with alpha- and beta- adrenergic agonists is mediated by different mechanisms.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenergic alpha-Agonists - pharmacology
- Adrenergic beta-Agonists - pharmacology
- Animals - pharmacology
- Blood Glucose - metabolism
- Drug Administration Schedule - metabolism
- Drug Implants - metabolism
- Heart - drug effects
- Kinetics - drug effects
- Male - drug effects
- Myocardium - metabolism
- Rats - metabolism
- Rats, Inbred Strains - metabolism
- Receptor, Insulin - drug effects