Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Glader, C; Jeitler, R; Stanzer, S; Harbusch, N; Prietl, B; El-Heliebi, A; Selmani, A; Fröhlich, E; Mussbacher, M; Roblegg, E.
Investigation of nanostructured lipid carriers for fast intracellular localization screening using the Echo liquid handler.
Int J Pharm. 2024; 665:124698
Doi: 10.1016/j.ijpharm.2024.124698
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- El-Heliebi Amin
- Fröhlich Eleonore
- Prietl Barbara
- Stanzer Stefanie
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- Abstract:
- In the field of precision medicine, therapy is optimized individually for each patient, enhancing efficacy while reducing side effects. This involves the identification of promising drug candidates through high-throughput screening on human derived cells in culture. However, screening of drugs which have poor solubility or permeability remains challenging, especially when targeting intracellular components. Therefore, encapsulation of drugs into advanced delivery systems such as nanostructured lipid carries (NLC) becomes necessary. Here we show that the cellular uptake of NLC with different matrix compositions can be assessed in a high-throughput screening system based on acoustic droplet ejection (ADE) technology (Echo liquid handler). Our findings indicate that surface tension and viscosity of the NLC dispersions need to be tailored to enable a reliable ADE transfer. The automated NLC uptake studies indicated that the composition of the matrix, more specifically the amount of oleic acid, significantly influenced cellular uptake. The data obtained were corroborated by imaging based and spectral flow cytometry cellular uptake studies. These findings thus not only provide the basis for a screening tool to rapidly identify the efficacy of NLC uptake but also enable a next step toward precision high-throughput drug screening under consideration of an optimized drug delivery system.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Drug Carriers - chemistry
- Lipids - chemistry
- Nanostructures - chemistry
- High-Throughput Screening Assays - methods
- Drug Delivery Systems - methods
- Oleic Acid - chemistry
- Viscosity - administration & dosage
- Find related publications in this database (Keywords)
- High-throughput screening
- Precision medicine
- Nanomedicine
- Cellular uptake
- Acoustic liquid handling