Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Rinnerthaler, G; Hackl, H; Gampenrieder, SP; Hamacher, F; Hufnagl, C; Hauser-Kronberger, C; Zehentmayr, F; Fastner, G; Sedlmayer, F; Mlineritsch, B; Greil, R.
miR-16-5p Is a Stably-Expressed Housekeeping MicroRNA in Breast Cancer Tissues from Primary Tumors and from Metastatic Sites.
Int J Mol Sci. 2016; 17(2):
Doi: 10.3390/ijms17020156
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- Leading authors Med Uni Graz
- Rinnerthaler Gabriel
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- Abstract:
- For quantitative microRNA analyses in formalin-fixed paraffin-embedded (FFPE) tissue, expression levels have to be normalized to endogenous controls. To investigate the most stably-expressed microRNAs in breast cancer and its surrounding tissue, we used tumor samples from primary tumors and from metastatic sites. MiRNA profiling using TaqMan(®) Array Human MicroRNA Cards, enabling quantification of 754 unique human miRNAs, was performed in FFPE specimens from 58 patients with metastatic breast cancer. Forty-two (72%) samples were collected from primary tumors and 16 (28%) from metastases. In a cross-platform analysis of a validation cohort of 32 FFPE samples from patients with early breast cancer genome-wide microRNA expression analysis using SurePrintG3 miRNA (8 × 60 K)(®) microarrays from Agilent(®) was performed. Eleven microRNAs could be detected in all samples analyzed. Based on NormFinder and geNorm stability values and the high correlation (rho ≥ 0.8) with the median of all measured microRNAs, miR-16-5p, miR-29a-3p, miR-126-3p, and miR-222-3p are suitable single gene housekeeper candidates. In the cross-platform validation, 29 human microRNAs were strongly expressed (mean log2-intensity > 10) and 21 of these microRNAs including miR-16-5p and miR-29a-3p were also stably expressed (CV < 5%). Thus, miR-16-5p and miR-29a-3p are both strong housekeeper candidates. Their Normfinder stability values calculated across the primary tumor and metastases subgroup indicate that miR-29a-3p can be considered as the strongest housekeeper in a cohort with mainly samples from primary tumors, whereas miR-16-5p might perform better in a metastatic sample enriched cohort.
- Find related publications in this database (using NLM MeSH Indexing)
- Breast Neoplasms - genetics, pathology
- Female - administration & dosage
- Gene Expression - administration & dosage
- Gene Expression Profiling - methods, standards
- Genes, Essential - administration & dosage
- Humans - administration & dosage
- MicroRNAs - genetics
- Neoplasm Metastasis - administration & dosage
- Oligonucleotide Array Sequence Analysis - methods, standards
- Find related publications in this database (Keywords)
- miR-16
- miR-16-5p
- microRNA
- endogenous control
- housekeeper
- control
- breast cancer