Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kicheeva, AG; Sushko, ES; Bondarenko, LS; Baimuratova, RK; Kydralieva, KA; Schwaminger, SP; Prassl, R; Tropskaya, NS; Dzhardimalieva, GI; Smirnykh, DV; Martynova, AA; Kudryasheva, NS.
Cytotoxic and radical activities of metal-organic framework modified with iron oxide: Biological and physico-chemical analyses.
Chem Biol Interact. 2024; 399:111150
Doi: 10.1016/j.cbi.2024.111150
Web of Science
PubMed
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FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Prassl Ruth
- Schwaminger Sebastian
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- Abstract:
- Metal-organic framework (MOF) modified with iron oxide, Fe3O4-MOF, is a perspective drug delivery agent, enabling magnetic control and production of active hydroxyl radicals, •OH, via the Fenton reaction. This paper studies cytotoxic and radical activities of Fe-containing nanoparticles (NPs): Fe3O4-MOF and its components - bare Fe3O4 and MOF (MIL-88B). Luminous marine bacteria Photobacteriumphosphoreum were used as a model cellular system to monitor bioeffects of the NPs. Neither the NPs of Fe3O4-MOF nor MOF showed cytotoxic effects in a wide range of concentrations (<10 mg/L); while Fe3O4 was toxic at >3·10-3 mg/L. The NPs of Fe3O4 did not affect the bacterial bioluminescence enzymatic system; their toxic effect was attributed to cellular membrane processes. The integral content of reactive oxygen species (ROS) was determined using a chemiluminescence luminol assay. Bacteria mitigated excess of ROS in water suspensions of Fe3O4-MOF and MOF, maintaining bioluminescence intensity closer to the control; this resulted in low toxicity of these NPs. We estimated the activity of •OH radicals in the NPs samples with physical and chemical methods - spin capture technology (using electron paramagnetic resonance spectroscopy) and methylene blue degradation. Physico-chemical interpretation of cellular responses is provided in terms of iron content, iron ions release and •OH radical production.
- Find related publications in this database (using NLM MeSH Indexing)
- Metal-Organic Frameworks - chemistry, pharmacology
- Photobacterium - drug effects
- Ferric Compounds - chemistry
- Hydroxyl Radical - chemistry, metabolism
- Reactive Oxygen Species - metabolism
- Electron Spin Resonance Spectroscopy - administration & dosage
- Cell Survival - drug effects
- Find related publications in this database (Keywords)
- Metal organic frameworks
- Magnetite nanoparticles
- Reactive oxygen species
- Toxicity
- Bacteria bioluminescence assay
- Ferroptosis