Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Greilberger, J; Stiegler, P; Greilberger, M; Wintersteiger, R.
The Influence of N-Acetyl-selenomethionine on Two RONS-Generating Cancer Cell Lines Compared to N-Acetyl-methionine.
Cells. 2024; 13(11):
Doi: 10.3390/cells13110937
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- Führende Autor*innen der Med Uni Graz
- Greilberger Joachim
- Co-Autor*innen der Med Uni Graz
- Greilberger Michaela
- Stiegler Philipp
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- Abstract:
- UNLABELLED: N-acetyl-selenomethionine (NASeLM), a representative of the selenium compounds, failed to convince in clinical studies and cell cultures that it neither inhibits cancer growth nor has a chemoprotective effect. This study aims to find out whether NASeLM shows a growth-inhibiting property compared to the carrier substance N-Acetyl-L-methionine (NALM) on two different cancer cells, namely Jurkat cells and MTC-SK cells. METHODS: Jurkat and MTC-SK cells were cultured in the absence or presence of varying concentrations (0-500 µg/mL) of NASeLM and NALM solutions. After 0, 24, 48, and 72 h, mitochondrial activity, cancer cell membrane CP levels, cell growth, and caspase-3 activity were assessed in aliquots of Jurkat and MTC-SK cells. RESULTS: Both substances, NASeLM and NALM, were similarly able to inhibit cell growth and mitochondrial activity of Jurkat cells in a concentration-dependent and time-dependent manner up to 70%. Only the determination of caspase activity showed that only NASeLM was able to increase this to almost 40% compared to the control as well as the same lack of NALM. However, the experiments on MTC-SK cells showed a clear difference in favor of NASeLM compared to NALM. While NASeLM was able to reduce cell growth to up to 55%, the same amount of NALM was only at around 15%, which turned out to be highly significant (p < 0.001). The same could also be measured for the reduction in MTC-SK mitochondrial activity. Time dependence could also be recognized: the longer both substances, NASeLM and NALM, were incubated, the higher the effect on cell growth and mitochondrial activity, in favour of NASeLM. Only NASeLM was able to increase caspase-3 activity in MTC-SK cells: at 250 µg/mL NASeLM, caspase-3 activity increased significantly to 28% after 24 and 48 h compared to the control (14%) or the same NALM concentration (14%). After 72 h, this could still increase to 37%. A further increase in the NASeLM concentration did not result in higher caspase-3 activity. CONCLUSION: NASeLM could clearly increase caspase-3 activity in both cell types, Jurkat or MTC-SK cells, and thus induce cell death. NALM and NASeLM showed a reduction in cell growth and mitochondrial activity in both cell lines: While NALM and NASeLM showed almost identical measurements on Jurkat cells, NASeLM was much more effective on MTC-SK than the non-selenium-containing carrier, indicating that it has additional anti-chemoprotective effects.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Selenomethionine - pharmacology
- Jurkat Cells - administration & dosage
- Methionine - analogs & derivatives, pharmacology, metabolism
- Cell Proliferation - drug effects
- Mitochondria - metabolism, drug effects
- Caspase 3 - metabolism
- Cell Line, Tumor - administration & dosage
- Apoptosis - drug effects
- Find related publications in this database (Keywords)
- N-acetyl-selenomethionine (NASeLM)
- N-Acetyl-L-methionine (NALM)
- T-cell leukemia cells (Jurkat cells)
- medullary thyroid carcinoma cells (MTC-SK)
- caspase-3 activity
- cell growth
- mitochondrial activity