Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Groschup, B; Calandra, GM; Raitmayr, C; Shrouder, J; Llovera, G; Zaki, AG; Burgstaller, S; Bischof, H; Eroglu, E; Liesz, A; Malli, R; Filser, S; Plesnila, N.
Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo.
Sci Rep. 2024; 14(1): 13753
Doi: 10.1038/s41598-024-62993-1
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- Co-Autor*innen der Med Uni Graz
- Bischof Helmut
- Burgstaller Sandra
- EROGLU Emrah
- Malli Roland
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- Abstract:
- Neuronal activity is accompanied by a net outflow of potassium ions (K+) from the intra- to the extracellular space. While extracellular [K+] changes during neuronal activity are well characterized, intracellular dynamics have been less well investigated due to lack of respective probes. In the current study we characterized the FRET-based K+ biosensor lc-LysM GEPII 1.0 for its capacity to measure intracellular [K+] changes in primary cultured neurons and in mouse cortical neurons in vivo. We found that lc-LysM GEPII 1.0 can resolve neuronal [K+] decreases in vitro during seizure-like and intense optogenetically evoked activity. [K+] changes during single action potentials could not be recorded. We confirmed these findings in vivo by expressing lc-LysM GEPII 1.0 in mouse cortical neurons and performing 2-photon fluorescence lifetime imaging. We observed an increase in the fluorescence lifetime of lc-LysM GEPII 1.0 during periinfarct depolarizations, which indicates a decrease in intracellular neuronal [K+]. Our findings suggest that lc-LysM GEPII 1.0 can be used to measure large changes in [K+] in neurons in vitro and in vivo but requires optimization to resolve smaller changes as observed during single action potentials.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Potassium - metabolism
- Neurons - metabolism
- Mice - administration & dosage
- Biosensing Techniques - methods
- Action Potentials - administration & dosage
- Cells, Cultured - administration & dosage
- Fluorescence Resonance Energy Transfer - methods
- Optogenetics - methods