Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ziegler, HP; Platzer, S; Haas, D; Galler, H; Schalli, M.
Protocol for inoculating bottled mineral water with bacteria to receive artificial contaminated water samples.
STAR Protoc. 2024; 5(2): 103026
Doi: 10.1016/j.xpro.2024.103026
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- Führende Autor*innen der Med Uni Graz
- Schalli Michael
- Ziegler Hans Peter
- Co-Autor*innen der Med Uni Graz
- Galler Herbert
- Haas Doris
- Platzer Sabine
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- Abstract:
- Here, we present a protocol for inoculating drinking water samples with a variety of pathogens or facultative pathogen bacteria. We describe steps for preparing bacterial solutions, inoculating mineral water bottles and other drinking water samples, filtration and incubation of the agar plates, and counting colony-forming unit per mL. We also detail procedures for determining selected chemical properties, such as anions and cations, which can also affect the bacterial growth. For complete details on the use and execution of this protocol, please refer to Schalli et al.1.