Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Benezeder, T; Bordag, N; Woltsche, J; Teufelberger, A; Perchthaler, I; Weger, W; Salmhofer, W; Gruber-Wackernagel, A; Painsi, C; Zhan, Q; El-Heliebi, A; Babina, M; Clark, R; Wolf, P.
Mast cells express IL17A, IL17F and RORC, are activated and persist with IL-17 production in resolved skin of patients with chronic plaque-type psoriasis.
Res Sq. 2024;
Doi: 10.21203/rs.3.rs-3958361/v1
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PubMed
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- Autor*innen der Med Uni Graz:
- Benezeder Theresa Helena
- Bordag Natalie
- El-Heliebi Amin
- Gruber-Wackernagel Alexandra
- Painsi Clemens
- Perchthaler Isabella
- Salmhofer Wolfgang
- Teufelberger Andrea Renate
- Weger Wolfgang
- Wolf Peter
- Woltsche Johannes Nikolaus
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- Abstract:
- Little is known about IL-17 expression in psoriasis and the actual cellular source of IL-17 remains incompletely defined. We show that high numbers of IL-17 + mast cells persisted in resolved lesions after treatment (anti-IL-17A, anti-IL-23, UVB or topical dithranol) and correlated inversely with the time span in remission. IL-17 + mast cells were found in T cell-rich areas and often close to resident memory T cells (Trm) in active psoriasis and resolved lesional skin. Digital cytometry by deconvolution of RNA-seq data showed that activated mast cells were increased in psoriatic skin, while resting mast cells were almost absent and both returned to normal levels after treatment. When primary human skin mast cells were stimulated with T cell cytokines (TNFα, IL-22 and IFNγ), they responded by releasing more IL-17A, as measured by ELISA. In situ mRNA detection using padlock probes specific for transcript variants of IL17A, IL17F, and RORC (encoding the Th17 transcription factor RORγt) revealed positive mRNA signals for IL17A, IL17F, and RORCin tryptase + cells, demonstrating that mast cells have the transcriptional machinery to actively produce IL-17. Mast cells thus belong to the center of the IL-23/IL-17 axis and high numbers of IL-17 + mast cells predict an earlier disease recurrence.