Medizinische Universität Graz - Research portal
Selected Publication:
Stünzner, D; Hubalek, Z; Halouzka, J; Postic, D; Pierer, K; Marth, E.
Prevalence of Borrelia burgdorferi s.I. in Ixodes ricinus ticks from Styria (Austria) and species identification by PCR-RFLP analysis.
Zentralbl Bakteriol. 1998; 288(4):471-478
Doi: 10.1016/S0934-8840(98)80063-7
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- Co-authors Med Uni Graz
- Marth Egon
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- Abstract:
- A total of 1163 I. ricinus ticks were collected in 3 different regions (15 localities) in Styria (Austria) in June 1997 and examined for the presence of spirochetes by dark field microscopy. The mean infection rate was 20.8%. Among 310 adults, 24.2% were positive and among 853 nymphs, 19.6% were positive. All 15 collection areas were shown to harbour infected nymphs with a positivity rate ranging from 5.8% (3/52) to 32.1% (18/56). Isolation attempts in BSKII medium resulted in 29 isolates. Species identification by PCR-RFLP analysis revealed 16 strains of B. garinii, 10 strains of B. afzelii and 2 strains of B. burgdorferi s. s. One isolate showed a mixed population of B. garinii and B. afzelii. In two collection areas, all three major Borrelia species were shown to be present in the tick population.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Arachnid Vectors - microbiology
- Austria - microbiology
- Bacterial Typing Techniques - microbiology
- Borrelia burgdorferi Group - classification
- Female - classification
- Genes, rRNA - classification
- Ixodes - growth and development
- Male - growth and development
- Nymph - microbiology
- Polymerase Chain Reaction - methods
- Polymorphism, Restriction Fragment Length - methods
- RNA, Ribosomal, 23S - genetics
- RNA, Ribosomal, 5S - genetics
- Research Support, Non-U.S. Gov't - genetics