Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kupper, N; Pritz, E; Siwetz, M; Guettler, J; Huppertz, B.
Ex Vivo Placental Explant Flow Culture - Mimicking the Dynamic Conditions In Utero.
J Vis Exp. 2023;
Doi: 10.3791/65919
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Huppertz Berthold
- Kupper Nadja Julia
- Co-Autor*innen der Med Uni Graz
- Güttler Jacqueline
- Siwetz Monika
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- Abstract:
- The existing ex vivo placental explant culture models are primarily grounded in static culture systems using well plates. However, these models inadequately reflect the dynamic in utero setting, where the placenta encounters constant slight shear stress due to plasma or blood flow. To address this limitation, a flow culture system has been devised to bring ex vivo placental explant cultivation closer to the in utero flow conditions experienced within the maternal body. Within this approach, placental explants are cultivated in a sequence of five interconnected flow chambers. This setting maintains physiological oxygen concentrations and a consistent flow rate. The collected data reveals that under flow conditions, the preservation of tissue morphology exhibits notable enhancement compared to conventional static methods. This innovative technique introduces a straightforward means of ex vivo placental explant culture, offering a more faithful representation of the dynamic in vivo environment. Moreover, this study introduces new possibilities for investigating the functional dynamics of the feto-maternal interface. By embracing feasible dynamic methodologies, a deeper comprehension of placental biology is facilitated, underscoring its relevance for maternal-fetal health.
- Find related publications in this database (using NLM MeSH Indexing)
- Pregnancy - administration & dosage
- Female - administration & dosage
- Humans - administration & dosage
- Placenta - administration & dosage
- Data Collection - administration & dosage
- Fetus - administration & dosage
- Pelvis - administration & dosage
- Plasma - administration & dosage