Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pirker, T; Pferschy-Wenzig, EM; Bampali, E; Bochkov, V; Bauer, R.
Glycolipid-enriched fraction of Osmanthus fragrans inhibits LPS-induced expression of inflammatory genes, COX-2, E-selectin, and Interleukin-8.
J Ethnopharmacol. 2023; 309: 116328
Doi: 10.1016/j.jep.2023.116328
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Pirker Teresa Marie
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- Abstract:
- ETHNOPHARMACOLOGICAL RELEVANCE: Osmanthus fragrans Lour. is a small ornamental tree native to the Southeastern parts of China. It is mainly cultivated because of its characteristic fragrance, and used in the food and perfume industry. Besides, its flowers are used in traditional Chinese medicine to treat a variety of diseases including those related to inflammation. AIM OF THE STUDY: The aim of the study was to investigate in more detail the anti-inflammatory properties of O. fragrans flowers, and to characterize their active principles and mechanisms of action. MATERIALS AND METHODS: O. fragrans flowers were successively extracted with n-hexane, dichloromethane and methanol. The extracts were further fractionated by chromatographic separation. COX-2 mRNA expression in PMA-differentiated, LPS-stimulated THP-1 cells was used as lead assay for activity-guided fractionation. The most potent fraction was chemically analyzed by LC-HRMS. The pharmacological activity was also evaluated in other inflammation-related in-vitro models, such as analysis of IL-8 secretion and E-selectin expression in HUVECtert cells and selective inhibition of COX-isoenzymes. RESULTS: n-Hexane and dichloromethane extracts of O. fragrans flowers significantly inhibited COX-2 (PTGS2) mRNA expression. Additionally, both extracts inhibited COX-2 enzyme activity, whereas COX-1 enzyme activity was affected to a significantly lower extent. Fractionation of the extracts led to a highly active, glycolipid-containing fraction. In total, 10 glycolipids were tentatively annotated by LC-HRMS. This fraction also inhibited LPS-induced COX-2 mRNA expression, IL-8 secretion and E-selectin expression. The effects were limited to LPS-induced inflammation and not observed when inflammatory genes were induced by TNF-α, IL-1β or FSL-1. Since all these inducers of inflammation act via different receptors, it is likely that the fraction interferes with the binding of LPS to the TLR4-receptor, which mediates pro-inflammatory effects of LPS. CONCLUSION: Taken together, the results demonstrate the anti-inflammatory potential of O. fragrans flower extracts in general, and of the glycolipid-enriched fraction in particular. The effects of glycolipid-enriched fraction are potentially mediated via the inhibition of the TLR4 receptor complex.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Interleukin-8 - genetics
- Cyclooxygenase 2 - genetics
- Plant Extracts - therapeutic use
- Lipopolysaccharides - toxicity
- Glycolipids - administration & dosage
- E-Selectin - genetics
- Methylene Chloride - adverse effects
- Anti-Inflammatory Agents - therapeutic use
- Inflammation - chemically induced, drug therapy, prevention & control
- RNA, Messenger - genetics
- Find related publications in this database (Keywords)
- Osmanthus fragrans
- COX-2 expression
- Inflammation
- Glycolipids
- Digalactosyl-diacylglycerol
- Monogalactosyl-monoacylglycerol