Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Greilberger, J; Wang, X; Ledinski, G; Chen, Q; Jürgens, G.
Presence of aldehydic epitopes on a minor low-density lipoprotein fraction.
Free Radic Biol Med. 1999; 26(11-12):1489-1494
Doi: 10.1016%2FS0891-5849%2899%2900008-8
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- Führende Autor*innen der Med Uni Graz
- Greilberger Joachim
- Jürgens Günther
- Co-Autor*innen der Med Uni Graz
- Ledinski Gerhard
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- Abstract:
- A more negatively charged low-density lipoprotein (LDL), named minor LDL (mi-LDL), was separated by ionic exchange chromatography and further characterized. This mi-LDL contained lower amounts of polyunsaturated fatty acids, alpha- or gamma- tocopherol, but higher amounts of lipid hydroperoxides than the major LDL fraction (ma-LDL). We show here for the first time that apoB of mi-LDL is modified by lipid peroxidation products, such as 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA). Using polyclonal antibodies, generated against 4-HNE- or MDA-LDL and apoB, the ratio of 4-HNE- or MDA-derived epitopes to apoB of mi-LDL and ma-LDL was estimated by means of a solid phase fluorescence immunoassay. The ratio of 4-HNE-derived epitopes to apoB on mi-LDL was fourfold higher, while the ratio of MDA-derived epitopes to apoB was twofold higher, compared with the ratios obtained with ma-LDL. In a competition assay with mi- and ma-LDL, only mi-LDL was an effective competitor to inhibit the immunoreaction of anti-4-HNE-LDL with 4-HNE-LDL (by 24%) and of anti-MDA-LDL with MDA-LDL (by 10%).
- Find related publications in this database (using NLM MeSH Indexing)
- Aldehydes - immunology
- Antioxidants - metabolism
- Blood Donors - metabolism
- Chemical Fractionation - metabolism
- Chromatography, Ion Exchange - metabolism
- Electrochemistry - metabolism
- Epitopes - metabolism
- Fatty Acids - blood
- Fluorometry - blood
- Free Radicals - blood
- Humans - blood
- Immunoassay - methods
- Lipid Peroxides - blood
- Lipoproteins, LDL - immunology
- Malondialdehyde - immunology
- Find related publications in this database (Keywords)
- Free Radicals
- Low-Density Lipoprotein
- Atherosclerosis
- Oxidation of Low-Density Lipoprotein