Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Babalola, JA; Lang, MGLA; George, M; Stracke, A; Tam-Amersdorfer, C; Itxaso, I; Lucija, D; Tadic, J; Schilcher, I; Loeffler, T; Flunkert, S; Prokesch, M; Leitinger, G; Lass, A; Hutter-Paier, B; Panzenboeck, U; Hoefler, G.
Astaxanthin enhances autophagy, amyloid beta clearance and exerts anti-inflammatory effects in in vitro models of Alzheimer's disease-related blood brain barrier dysfunction and inflammation
BRAIN RES. 2023; 1819: 148518
Doi: 10.1016/j.brainres.2023.148518
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Babalola Joshua Adekunle
- Höfler Gerald
- Co-authors Med Uni Graz
- George Meekha
- Leitinger Gerd
- Löffler Tina
- Panzenboeck Ute
- Schilcher Irene Rosa Maria
- Stracke Anika
- Tadic Jelena
- Tam-Amersdorfer Carmen
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- Abstract:
- Defective degradation and clearance of amyloid-beta as well as inflammation per se are crucial players in the pathology of Alzheimer's disease (AD). A defective transport across the blood-brain barrier is causative for amyloid-beta (A beta) accumulation in the brain, provoking amyloid plaque formation. Using primary porcine brain capillary endothelial cells and murine organotypic hippocampal slice cultures as in vitro models of AD, we investigated the effects of the antioxidant astaxanthin (ASX) on A beta clearance and neuroinflammation. We report that ASX enhanced the clearance of misfolded proteins in primary porcine brain capillary endothelial cells by inducing autophagy and altered the A beta processing pathway. We observed a reduction in the expression levels of intracellular and secreted amyloid precursor protein/A beta accompanied by an increase in ABC transporters ABCA1, ABCG1 as well as low density lipoprotein receptor-related protein 1 mRNA levels. Furthermore, ASX treatment increased autophagic flux as evidenced by increased lipidation of LC3B-II as well as reduced protein expression of phosphorylated S6 ribosomal protein and mTOR. In LPS-stimulated brain slices, ASX exerted anti-inflammatory effects by reducing the secretion of inflammatory cytokines while shifting microglia polarization from M1 to M2
- Find related publications in this database (Keywords)
- Blood-brain barrier
- Amyloid beta
- Autophagy
- Inflammation
- Astaxanthin