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Lercherhartlieb, A; Pertl, B; Häusler, M; Pieber, D; Huttner, U; Vadon, M; Winter, R.
Prenatal Determination of Fetal Rhd Type by Polymerase-Chain-Reaction on Amniocytes - Initial Clinical-Experiences
GEBURTSH FRAUENHEILK 1998 58: 575-579. Doi: 10.1055/s-2007-1022764
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Leading authors Med Uni Graz: Pertl Barbara
Co-authors Med Uni Graz: Haeusler Martin; Pieber Doris; Winter Raimund
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Abstract:: Purpose: Prenatal fetal RhD typing by means of polymerase chain reaction on amniotic fluid cells is a valuable tool for the management of alloimmunised pregnant women whose partners are known to be heterozygous for the RhD gene. Our purpose was to establish the reliability of fetal RhD typing using PCR and to assess the possible clinical application on this molecular technique. Material and Method: Analysis of amniotic fluid samples (n=35) and adult blood samples (n=83) was performed using two sets of primers to amplify different exons of both RhD and RhCE genes. Results: In all cases there was a complete agreement between the results of PCR and serologic typing. This technique was applied to amniotic fluid samples obtained by amniocentesis from five RhD-negative women with known RhD sensitisation and heterozygous partners. Knowledge of the fetal RhD status enabled rational clinical management in these pregnancies. Conclusion: RhD gene analysis is a rapid and reliable technique that provides an accurate fetal genotype which is potentially able to be a great help in the prenatal care of alloimmunised pregnant women.