Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Etschmaier, V; Üçal, M; Lohberger, B; Absenger-Novak, M; Kolb, D; Weinberg, A; Schäfer, U.
Disruption of Endochondral Ossification and Extracellular Matrix Maturation in an Ex Vivo Rat Femur Organotypic Slice Model Due to Growth Plate Injury.
Cells. 2023; 12(13): 1687
Doi: 10.3390/cells12131687
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- Führende Autor*innen der Med Uni Graz
- Etschmaier Vanessa
- Schäfer Ute
- Co-Autor*innen der Med Uni Graz
- Absenger-Novak Markus
- Kolb Dagmar
- Lohberger Birgit
- Ücal Muammer
- Weinberg Annelie-Martina
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- Abstract:
- Postnatal bone fractures of the growth plate (GP) are often associated with regenerative complications such as growth impairment. In order to understand the underlying processes of trauma-associated growth impairment within postnatal bone, an ex vivo rat femur slice model was developed. To achieve this, a 2 mm horizontal cut was made through the GP of rat femur prior to the organotypic culture being cultivated for 15 days in vitro. Histological analysis showed disrupted endochondral ossification, including disordered architecture, increased chondrocyte metabolic activity, and a loss of hypertrophic zone throughout the distal femur. Furthermore, altered expression patterns of Col2α1, Acan, and ColX, and increased chondrocyte metabolic activity in the TZ and MZ at day 7 and day 15 postinjury were observed. STEM revealed the presence of stem cells, fibroblasts, and chondrocytes within the injury site at day 7. In summary, the findings of this study suggest that the ex vivo organotypic GP injury model could be a valuable tool for investigating the underlying mechanisms of GP regeneration post-trauma, as well as other tissue engineering and disease studies.
- Find related publications in this database (using NLM MeSH Indexing)
- Rats - administration & dosage
- Animals - administration & dosage
- Osteogenesis - administration & dosage
- Salter-Harris Fractures - metabolism, pathology
- Chondrocytes - metabolism
- Extracellular Matrix - metabolism
- Femur - pathology
- Find related publications in this database (Keywords)
- bone metabolism
- injury repair
- organotypic culture
- endochondral ossification