Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Balazs, I; Horvath, A; Heschl, B; Khalil, M; Enzinger, C; Stadlbauer, V; Seifert-Held, T.
Anti-CD20 treatment and neutrophil function in central nervous system demyelinating diseases.
J Neuroimmunol. 2023; 381:578136
Doi: 10.1016/j.jneuroim.2023.578136
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Balazs Irina
- Seifert-Held Thomas
- Co-Autor*innen der Med Uni Graz
- Enzinger Christian
- Horvath Angela
- Khalil Michael
- Stadlbauer-Köllner Vanessa
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- INTRODUCTION: A contribution of neutrophil granulocytes to the pathogenesis of multiple sclerosis (MS) and neuromyelitis optica spectrum disorders (NMOSD) is recognized. Anti-CD20 treatments applied in these diseases are associated with infectious complications and neutropenia. No data is available about functional characteristics of neutrophils obtained from patients with anti-CD20 treatments. METHODS: In neutrophils isolated from 13 patients with anti-CD20 treatment (9 MS, 4 NMOSD), 11 patients without anti-CD20 treatment (9 MS, 2 NMOSD) and 5 healthy controls, we analyzed chemotaxis, production of reactive oxygen species (ROS), phagocytosis, and formation of neutrophil extracellular traps (NET) in vitro. RESULTS: Chemotaxis and ROS production were found unchanged between patients with and without anti-CD20 treatment or between patients and healthy controls. We found a higher proportion of non-phagocytosing cells in patients without anti-CD20 treatment compared to patients with anti-CD20 treatment and healthy controls. As compared to healthy controls, a higher proportion of neutrophils from patients without anti-CD20 treatments underwent NET formation, either unstimulated or stimulated with phorbol 12-myristate 3-acetate for 3 h. In about half of patients with anti-CD20 treatment (n = 7), NET formation of unstimulated neutrophils occurred already within 20 min of incubation. This was not observed in patients without anti-CD20 treatment and healthy controls. CONCLUSION: Anti-CD20 treatment in MS and NMOSD patients does not alter chemotaxis and ROS production of neutrophils in vitro but might restore their impaired phagocytosis in these diseases. Our study reveals a predisposition to early NET formation in vitro of neutrophils obtained from patients with anti-CD20 treatment. This may contribute to associated risks of neutropenia and infections.
- Find related publications in this database (using NLM MeSH Indexing)
- Humans - administration & dosage
- Neutrophils - administration & dosage
- Reactive Oxygen Species - administration & dosage
- Neutropenia - administration & dosage
- Central Nervous System Diseases - administration & dosage
- Multiple Sclerosis - administration & dosage
- Central Nervous System - administration & dosage
- Find related publications in this database (Keywords)
- Multiple sclerosis
- NMODS
- Rituximab
- Neutrophils
- NET formation