Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Meindl, C; Absenger-Novak, M; Jeitler, R; Roblegg, E; Frohlich, E.
Assessment of Carbon Nanotubes on Barrier Function, Ciliary Beating Frequency and Cytokine Release in In Vitro Models of the Respiratory Tract
NANOMATERIALS-BASEL. 2023; 13(4): 682
Doi: 10.3390/nano13040682
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- Führende Autor*innen der Med Uni Graz
- Fröhlich Eleonore
- Meindl Claudia
- Co-Autor*innen der Med Uni Graz
- Absenger-Novak Markus
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- Abstract:
- The exposure to inhaled carbon nanotubes (CNT) may have adverse effects on workers upon chronic exposure. In order to assess the toxicity of inhaled nanoparticles in a physiologically relevant manner, an air-liquid interface culture of mono and cocultures of respiratory cells and assessment in reconstructed bronchial and alveolar tissues was used. The effect of CNT4003 reference particles applied in simulated lung fluid was studied in bronchial (Calu-3 cells, EpiAirway (TM) and MucilAir (TM) tissues) and alveolar (A549 +/-THP-1 and EpiAlveolar (TM) +/-THP-1) models. Cytotoxicity, transepithelial electrical resistance, interleukin 6 and 8 secretion, mucociliary clearance and ciliary beating frequency were used as readout parameters. With the exception of increased secretion of interleukin 6 in the EpiAlveolar (TM) tissues, no adverse effects of CNT4003 particles, applied at doses corresponding to the maximum estimated lifetime exposure of workers, in the bronchial and alveolar models were noted, suggesting no marked differences between the models. Since the doses for whole-life exposure were applied over a shorter time, it is not clear if the interleukin 6 increase in the EpiAlveolar (TM) tissues has physiological relevance.
- Find related publications in this database (Keywords)
- carbon nanotubes
- toxicity
- in vitro models
- respiratory tract
- bronchial epithelium
- alveolar epithelium
- ciliary beating frequency