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SHR Neuro Cancer Cardio Lipid Metab Microb

Grill, B; Pavkov-Keller, T; Grininger, C; Darnhofer, B; Gruber, K; Hall, M; Schwab, H; Steiner, K.
Engineering TM1459 for Stabilisation against Inactivation by Amino Acid Oxidation
CHEM-ING-TECH. 2023; Doi: 10.1002/cite.202200176
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Co-authors Med Uni Graz: Darnhofer Barbara
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Abstract:: Oxidative alkene cleavage is a highly interesting reaction to obtain aldehydes and ketones. The Mn-dependent protein TM1459 from Thermotoga maritima can catalyse alkene cleavage of styrene derivatives in the presence of tert-butyl hydroperoxide. Despite the high thermal stability of the enzyme, it gets inactivated during the reaction. The data reported here indicate that auto-oxidation is responsible for the low stability of TM1459 in the oxidative environment required for the alkene cleavage reaction. By targeting the exchange of residues prone to oxidation, this phenomenon was successfully prevented. Importantly, the stability to oxidation conveyed by the amino acid exchanges led to increased enzyme activity. However, the exchanges resulted in slightly modified positions of two of the four metal-binding amino acids, thereby strongly impacting metal binding.
Find related publications in this database (Keywords): Alkene cleavage; Amino acid oxidation; Cupin; Stability