Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Knittelfelder, OL; Weberhofer, BP; Eichmann, TO; Kohlwein, SD; Rechberger, GN.
A versatile ultra-high performance LC-MS method for lipid profiling.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014; 951-952: 119-28.
Doi: 10.1016/j.jchromb.2014.01.011
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- Co-Autor*innen der Med Uni Graz
- Eichmann Thomas
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- Abstract:
- A new UPLC-based untargeted lipidomic approach using a qTOF hybrid mass spectrometer is introduced. The applied binary gradient enables separations of lipid species including constitutional isomeric compounds and low abundant lipid classes such as phosphatidic acid (PA). Addition of phosphoric acid to the solvents improves peak shapes for acidic phospholipids. MS(E) scans allow simultaneous acquisition of full scan data and collision induced fragmentation to improve identification of lipid classes and to obtain structural information. The method was used to investigate the lipidome of yeast.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Chromatography, High Pressure Liquid - methods
- Glycerophospholipids - administration & dosage
- Lipids - analysis, chemistry
- Mass Spectrometry - methods
- Mice - administration & dosage
- Muscles - chemistry
- Phosphoric Acids - administration & dosage
- Yeasts - chemistry
- Find related publications in this database (Keywords)
- Lipidomics
- Ultra-performance liquid chromatography
- Mass spectrometry
- Neutral lipids
- Glycerophospholipids