Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kohl, P; Zingl, FG; Eichmann, TO; Schild, S.
Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses.
Methods Mol Biol. 2018; 1839: 117-134.
Doi: 10.1007/978-1-4939-8685-9_11
PubMed
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- Co-authors Med Uni Graz
- Eichmann Thomas
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- Abstract:
- Outer membrane vesicles (OMVs) are naturally secreted from the bacterial cell surface and therefore localized in the cell-free supernatant of bacterial cultures. Here we describe methods for crude and density gradient-purified OMV isolation and protocols for control analyses for protein profiling (SDS-PAGE), detection of indicator proteins (immunoblot analysis), lipid profiling (lipid extraction and LC-MS analysis), vesicle size determination (NanoSight), rough estimation of biomass (TrayCell™), as well as quantifications of defined OMV components, e.g., proteins (Bradford) and LPS (Purpald).
- Find related publications in this database (using NLM MeSH Indexing)
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- Secretory Vesicles - administration & dosage
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- Ultracentrifugation - administration & dosage