Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Collier, MP; Alderson, TR; de, Villiers, CP; Nicholls, D; Gastall, HY; Allison, TM; Degiacomi, MT; Jiang, H; Mlynek, G; Fürst, DO; van, der, Ven, PFM; Djinovic-Carugo, K; Baldwin, AJ; Watkins, H; Gehmlich, K; Benesch, JLP.
HspB1 phosphorylation regulates its intramolecular dynamics and mechanosensitive molecular chaperone interaction with filamin C.
Sci Adv. 2019; 5(5): eaav8421
Doi: 10.1126/sciadv.aav8421
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- Co-Autor*innen der Med Uni Graz
- Alderson Thomas Reid
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- Abstract:
- Mechanical force-induced conformational changes in proteins underpin a variety of physiological functions, typified in muscle contractile machinery. Mutations in the actin-binding protein filamin C (FLNC) are linked to musculoskeletal pathologies characterized by altered biomechanical properties and sometimes aggregates. HspB1, an abundant molecular chaperone, is prevalent in striated muscle where it is phosphorylated in response to cues including mechanical stress. We report the interaction and up-regulation of both proteins in three mouse models of biomechanical stress, with HspB1 being phosphorylated and FLNC being localized to load-bearing sites. We show how phosphorylation leads to increased exposure of the residues surrounding the HspB1 phosphosite, facilitating their binding to a compact multidomain region of FLNC proposed to have mechanosensing functions. Steered unfolding of FLNC reveals that its extension trajectory is modulated by the phosphorylated region of HspB1. This may represent a posttranslationally regulated chaperone-client protection mechanism targeting over-extension during mechanical stress.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Binding Sites - administration & dosage
- Filamins - genetics, physiology
- Heart - physiology
- Heat-Shock Proteins - genetics, physiology
- Humans - administration & dosage
- Male - administration & dosage
- Mice - administration & dosage
- Mice, Inbred C57BL - administration & dosage
- Mice, Knockout - administration & dosage
- Molecular Chaperones - genetics, physiology
- Mutation - administration & dosage
- Myocardium - metabolism
- Phosphorylation - administration & dosage
- Protein Denaturation - administration & dosage
- Protein Domains - administration & dosage
- Protein Folding - administration & dosage
- Protein Structure, Secondary - administration & dosage
- Recombinant Proteins - administration & dosage
- Stress, Mechanical - administration & dosage