Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Fodor, M; Lanser, L; Hofmann, J; Otarashvili, G; Pühringer, M; Cardini, B; Oberhuber, R; Resch, T; Weissenbacher, A; Maglione, M; Margreiter, C; Zelger, P; Pallua, JD; Öfner, D; Sucher, R; Hautz, T; Schneeberger, S.
Hyperspectral Imaging as a Tool for Viability Assessment During Normothermic Machine Perfusion of Human Livers: A Proof of Concept Pilot Study.
Transpl Int. 2022; 35: 10355
Doi: 10.3389/ti.2022.10355
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- Co-authors Med Uni Graz
- Sucher Robert
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- Abstract:
- Normothermic machine perfusion (NMP) allows for ex vivo viability and functional assessment prior to liver transplantation (LT). Hyperspectral imaging represents a suitable, non-invasive method to evaluate tissue morphology and organ perfusion during NMP. Liver allografts were subjected to NMP prior to LT. Serial image acquisition of oxygen saturation levels (StO2), organ hemoglobin (THI), near-infrared perfusion (NIR) and tissue water indices (TWI) through hyperspectral imaging was performed during static cold storage, at 1h, 6h, 12h and at the end of NMP. The readouts were correlated with perfusate parameters at equivalent time points. Twenty-one deceased donor livers were included in the study. Seven (33.0%) were discarded due to poor organ function during NMP. StO2 (p < 0.001), THI (p < 0.001) and NIR (p = 0.002) significantly augmented, from static cold storage (pre-NMP) to NMP end, while TWI dropped (p = 0.005) during the observational period. At 12-24h, a significantly higher hemoglobin concentration (THI) in the superficial tissue layers was seen in discarded, compared to transplanted livers (p = 0.036). Lactate values at 12h NMP correlated negatively with NIR perfusion index between 12 and 24h NMP and with the delta NIR perfusion index between 1 and 24h (rs = -0.883, p = 0.008 for both). Furthermore, NIR and TWI correlated with lactate clearance and pH. This study provides first evidence of feasibility of hyperspectral imaging as a potentially helpful contact-free organ viability assessment tool during liver NMP.
- Find related publications in this database (using NLM MeSH Indexing)
- Hemoglobins - administration & dosage
- Humans - administration & dosage
- Hyperspectral Imaging - administration & dosage
- Lactates - administration & dosage
- Liver - diagnostic imaging
- Organ Preservation - methods
- Perfusion - methods
- Pilot Projects - administration & dosage
- Find related publications in this database (Keywords)
- transplantation
- perfusion
- normothermic
- imaging
- liver
- hyperspectral
- machine