Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schweighofer, B; Testori, J; Sturtzel, C; Sattler, S; Mayer, H; Wagner, O; Bilban, M; Hofer, E.
The VEGF-induced transcriptional response comprises gene clusters at the crossroad of angiogenesis and inflammation.
Thromb Haemost. 2009; 102(3): 544-54.
Doi: 10.1160/TH08-12-0830
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Sattler Susanne
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- VEGF-A is the major trigger of vasculogenesis and physiologic angiogenesis. We have investigated to which extent the gene repertoire induced by VEGF-A in endothelial cells is distinct from that of other growth factors and inflammatory cytokines. Genes upregulated in human umbilical vein endothelial cells treated with VEGF, EGF or IL-1 were compared by microarray analysis and clusters characteristic for individual or combinations of inducers were defined. VEGF-A upregulated in comparison to EGF a five-fold larger gene repertoire, which surprisingly overlapped to 60% with the inflammatory repertoire of IL-1. As shown by real-time RT-PCR for selected genes, VEGF-induction was mostly mediated by VEGF receptor-2 and the capacity of VEGF-A to induce genes in common with IL-1 largely depended on activation of the calcineurin/NFAT pathway, since cyclosporin A inhibited this induction. Another angiogenic growth factor, bFGF, did not share a comparable induction of inflammatory genes, but partially induced a small group of genes in common with VEGF-A, which were not regulated by EGF. Thus, the data display that VEGF-A induces a distinct gene repertoire, which, contrasting with other growth factors such as EGF or bFGF, includes an inherent inflammatory component possibly contributing to the cross-regulation of angiogenesis and inflammation as further indicated by the VEGF-mediated induction of leukocyte adhesion. Furthermore, a small group of genes selectively induced by VEGF-A with potential importance for angiogenesis is defined.
- Find related publications in this database (using NLM MeSH Indexing)
- Calcineurin - metabolism
- Cyclosporine - metabolism
- Endothelial Cells - cytology
- Gene Expression Regulation - administration & dosage
- Humans - administration & dosage
- Inflammation - administration & dosage
- Interleukin-1 - metabolism
- Models, Genetic - administration & dosage
- Multigene Family - administration & dosage
- Neovascularization, Pathologic - administration & dosage
- Oligonucleotide Array Sequence Analysis - administration & dosage
- Reverse Transcriptase Polymerase Chain Reaction - administration & dosage
- Transcription, Genetic - administration & dosage
- Vascular Endothelial Growth Factor A - metabolism
- Vascular Endothelial Growth Factor Receptor-2 - metabolism
- Find related publications in this database (Keywords)
- VEGF-A
- endothelial cells
- angiogenesis
- inflammation
- gene repertoire