Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Malle, E; Sattler, W; Prenner, E; Leis, HJ; Karàdi, I; Knipping, G; Romics, L; Kostner, GM.
Platelet membrane fluidity in type IIA, type IIB and type IV hyperlipoproteinemia.
Atherosclerosis. 1991; 87(2-3):159-167
Doi: 10.1016/0021-9150(91)90018-X
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Malle Ernst
- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
- Leis Hans-Joerg
- Sattler Wolfgang
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Fluorescence spectroscopy, a very sensitive index for measuring the biophysical properties of living cell systems, was used to examine the structural order of intact, resting, gel-filtered platelets from hyperlipidemic subjects (n = 48, 25-70 years) and normolipemic subjects (n = 34, 19-68 years). Fluorescence anisotropy (r[s]), which is inversely related to membrane fluidity, was estimated using 3 different fluorescent dyes, DPH, TMA-DPH, and 6-AS, known to label different regions of biological membranes. Increased membrane fluidity was observed in type IIB (n = 24, 36-62 yrs; r[s] = 0.0692 +/- 0.09) and type IV (n = 10, 33-57 yrs; r[s] = 0.058 +/- 0.006) hyperlipidemics in comparison to type IIA (n = 14, 25-70 yrs; r[s] = 0.086 +/- 0.019) and control subjects (n = 24, 28-68 yrs; r[s] = 0.079 +/- 0.012). The temperature dependency of r[s]-DPH values was significantly different (P less than 0.01) in platelets from type IIB and type IV patients compared to type IIA and control subjects of similar age. A significant positive correlation (P less than 0.005) between membrane fluidity and age was found only in healthy control subjects (n = 34, 19-68 yrs). Despite significant (P less than 0.01) differences in plasma lipid concentrations in hyperlipidemic patients and controls, significant ex vivo relations between membrane fluidity and lipoprotein concentrations, free fatty acid distribution, and increased age were found only in healthy control subjects. Plasma levels of thromboxane as well as serum selenium concentrations did not significantly differ between hypercholesterolemic, hypertriglyceridemic, and control subjects.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Blood Platelets - physiology
- Female - physiology
- Fluorescence Polarization - physiology
- Humans - physiology
- Hyperlipoproteinemia Type II - blood
- Hyperlipoproteinemia Type IV - blood
- Male - blood
- Membrane Fluidity - blood
- Middle Aged - blood
- Spectrometry, Fluorescence - blood
- Find related publications in this database (Keywords)
- HYPERLIPOPROTEINEMIA
- TYPE-IIA
- TYPE-IIB
- TYPE-IV
- MEMBRANE FLUIDITY
- PLATELETS