Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bains, HK; Bawa, SR; Pabst, MA; Sehgal, S.
Plasma membrane alterations of maturing goat (Capra indicus) spermatozoa: lectin-binding and freeze-fracture study.
Cell Tissue Res. 1993; 271(1):159-168
Doi: 10.1007/BF00297554
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PubMed
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- Co-Autor*innen der Med Uni Graz
- Pabst Maria-Anna
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- Abstract:
- A qualitative and quantitative analysis of lectin-binding sites has been undertaken on spermatozoa recovered from different regions of the epididymis of the goat (Capra indicus) using fluorescein isothiocyanate-linked lectins (Bauhinia purpurea BPA, Concanavalin A Con A, Dolichos biflorus DBA, Maclura pomifera MPA, Arachis hypogaea or peanut agglutinin PNA, Glycine max or soyabean agglutinin SBA, Ulex europaeus UEA, and Triticum vulgaris or wheat-germ agglutinin WGA), in conjunction with scanning and transmission electron microscopy, and freeze-fracture techniques. Flow cytometric analysis has also been used to quantitize binding affinity. Spermatozoa from caput to cauda epididymidis show no significant variation in lectin-binding ability, but the samples removed from the corpus epididymidis contain a greater number of binding sites. The passage of spermatozoa through the epididymidis is accompanied by a redistribution of the plasma membrane lectin-receptors covering the sperm head and tail. Receptors for BPA, DBA, PNA and SBA are specifically restricted to the anterior region of the acrosome in caudal spermatozoa. Freeze-fracture replicas, examined to study changes in organisation of intramembranous particles of the plasma membrane during sperm maturation, reveal distinct changes in their distribution in the acrosome, post-acrosome and spermatozoon tail, especially in the corpus and cauda epididymidis.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Binding Sites -
- Carbohydrate Metabolism -
- Carbohydrate Sequence -
- Carbohydrates - chemistry
- Cell Membrane - metabolism
- Epididymis - cytology
- Freeze Fracturing - cytology
- Goats - anatomy and histology
- Lectins - metabolism
- Male - metabolism
- Microscopy, Electron - metabolism
- Molecular Sequence Data - metabolism
- Sperm Maturation - metabolism
- Spermatozoa - metabolism
- Find related publications in this database (Keywords)
- Epididymis
- Spermatozoa
- Lectins
- Intramembranous Particles
- Flow Cytometry
- Freeze-Fracturing
- Fluorescent Cytochemistry
- Goat
- Capra-Indicus