Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kittler, S; Ebner, J; Besleaga, M; Larsbrink, J; Darnhofer, B; Birner-Gruenberger, R; Schobesberger, S; Akhgar, CK; Schwaighofer, A; Lendl, B; Spadiut, O.
Recombinant Protein L: Production, Purification and Characterization of a Universal Binding Ligand.
J Biotechnol. 2022; 359:108-115
Doi: 10.1016/j.jbiotec.2022.10.002
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Darnhofer Barbara
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- Abstract:
- Protein L (PpL) is a universal binding ligand that can be used for the detection and purification of antibodies and antibody fragments. Due to the unique interaction with immunoglobulin light chains, it differs from other affinity ligands, like protein A or G. However, due to its current higher market price, PpL is still scarce in applications. In this study, we investigated the recombinant production and purification of PpL and characterized the product in detail. We present a comprehensive roadmap for the production of the versatile protein PpL in E. coli.
- Find related publications in this database (using NLM MeSH Indexing)
- Ligands - administration & dosage
- Chromatography, Affinity - administration & dosage
- Escherichia coli - genetics, metabolism
- Bacterial Proteins - metabolism
- Recombinant Proteins - metabolism
- Immunoglobulin Fragments - administration & dosage
- Immunoglobulin Light Chains - administration & dosage
- Protein Binding - administration & dosage
- Find related publications in this database (Keywords)
- Protein L
- E
- coli
- Recombinant production
- Bioreactor
- Affinity ligand