Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Avdili, A; Rohrhofer, L; Auer, M; Bernecker, C; Schlenke, P; Dorn, I.
Generation of the human erythroblast-derived iPSC line UBTi001-A
STEM CELL RES. 2022; 64: 102910
Doi: 10.1016/j.scr.2022.102910
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Avdili Afrim
- Co-Autor*innen der Med Uni Graz
- Auer Martina
- Bernecker Claudia
- Dorn Isabel
- Rohrhofer Lisa
- Schlenke Peter
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- Abstract:
- We performed reprogramming of human erythroblasts derived from CD34+ hematopoietic stem / progenitor cells of a healthy donor. CD34+ cells were differen-tiated in-vitro into a pure population of CD36+ erythroblasts and nucleofected with four episomal plasmids expressing SOX2, OCT3/4, KLF4, LIN28, L-MYC and TP53-shRNA. The established iPSC line showed normal karyotype. Pluripotency was confirmed by expression of pluripotency markers and in-vitro differentiation into tissues of all three germ layers. The UBTi001-A iPSC line might provide an attractive source for developmental research on human hematopoiesis and erythropoiesis.