Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bernecker, C; Matzhold, EM; Kolb, D; Avdili, A; Rohrhofer, L; Lampl, A; Trötzmüller, M; Singer, H; Oldenburg, J; Schlenke, P; Dorn, I.
Membrane Properties of Human Induced Pluripotent Stem Cell-Derived Cultured Red Blood Cells.
Cells. 2022; 11(16):
Doi: 10.3390/cells11162473
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- Führende Autor*innen der Med Uni Graz
- Bernecker Claudia
- Dorn Isabel
- Co-Autor*innen der Med Uni Graz
- Avdili Afrim
- Kolb Dagmar
- Lampl Annika
- Matzhold Eva-Maria
- Rohrhofer Lisa
- Schlenke Peter
- Trötzmüller Martin
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- Abstract:
- Cultured red blood cells from human induced pluripotent stem cells (cRBC_iPSCs) are a promising source for future concepts in transfusion medicine. Before cRBC_iPSCs will have entrance into clinical or laboratory use, their functional properties and safety have to be carefully validated. Due to the limitations of established culture systems, such studies are still missing. Improved erythropoiesis in a recently established culture system, closer simulating the physiological niche, enabled us to conduct functional characterization of enucleated cRBC_iPSCs with a focus on membrane properties. Morphology and maturation stage of cRBC_iPSCs were closer to native reticulocytes (nRETs) than to native red blood cells (nRBCs). Whereas osmotic resistance of cRBC_iPSCs was similar to nRETs, their deformability was slightly impaired. Since no obvious alterations in membrane morphology, lipid composition, and major membrane associated protein patterns were observed, reduced deformability might be caused by a more primitive nature of cRBC_iPSCs comparable to human embryonic- or fetal liver erythropoiesis. Blood group phenotyping of cRBC_iPSCs further confirmed the potency of cRBC_iPSCs as a prospective device in pre-transfusional routine diagnostics. Therefore, RBC membrane analyses obtained in this study underscore the overall prospects of cRBC_iPSCs for their future application in the field of transfusion medicine.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Differentiation - physiology
- Erythrocytes - metabolism
- Erythropoiesis - administration & dosage
- Humans - administration & dosage
- Induced Pluripotent Stem Cells - administration & dosage
- Prospective Studies - administration & dosage
- Find related publications in this database (Keywords)
- red blood cells
- induced pluripotent stem cells
- erythropoiesis
- membrane
- blood group antigen
- deformability
- osmotic resistance
- phospholipids
- cholesterol
- reticulocytes