Medizinische Universität Graz - Research portal
Selected Publication:
Rössler, A.
An ultrasensitive, nonisotopic immunoassay for hyaluronan using the streptavidin-biotin system.
Clin Chim Acta. 1998; 270(2):101-114
Doi: 10.1016%2FS0009-8981%2897%2900211-8
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- Leading authors Med Uni Graz
- Rössler Andreas
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- Abstract:
- A time-resolved fluoroimmunoassay for measuring hyaluronan concentrations in plasma and several biological fluids is described. The solid-phase immunoassay is based on the competition between aggregation of hyaluronan with the cartilage proteoglycan monomer, followed by binding of a monoclonal antibody to keratan sulfate of the proteoglycan and a biotinylated anti-mouse IgG. Fluorescence can be measured by a time-resolved fluorometer after binding of Eu(3+)-labelled streptavidin to the biotinylated IgG. The assay is precise and correlates well (r = 0.986) with the only established radioimmunoassay known. The results show that it is essential to perform a blank run without addition of proteoglycan, as endogenous proteoglycan disturbs the measurement and causes underestimation of plasma hyaluronan. The distinguishing feature of this assay is its extreme sensitivity (< 0.24 microgram/l of plasma). The mean analytical recovery after serial dilutions and addition was 100.3 and 101.3%, the within-assay and between-assay coefficients of variation were 3.67% and 7.02%, respectively.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies, Monoclonal -
- Binding, Competitive -
- Biotin -
- Cartilage - chemistry
- Chondroitin Sulfates - metabolism
- Fluoroimmunoassay - metabolism
- Humans - metabolism
- Hyaluronic Acid - blood
- Immunoassay - methods
- Keratan Sulfate - metabolism
- Liver Diseases - blood
- Proteoglycans - blood
- Radioimmunoassay - blood
- Sensitivity and Specificity - blood
- Streptavidin - blood