Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Radulović, S; Sunkara, S; Rachel, R; Leitinger, G.
Three-dimensional SEM, TEM, and STEM for analysis of large-scale biological systems.
Histochem Cell Biol. 2022;
Doi: 10.1007/s00418-022-02117-w
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- Führende Autor*innen der Med Uni Graz
- Leitinger Gerd
- Radulovic Snjezana
- Co-Autor*innen der Med Uni Graz
- Sunkara Sowmya
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- Abstract:
- A major aim in structural cell biology is to analyze intact cells in three dimensions, visualize subcellular structures, and even localize proteins at the best possible resolution in three dimensions. Though recently developed electron microscopy tools such as electron tomography, or three-dimensional (3D) scanning electron microscopy, offer great resolution in three dimensions, the challenge is that, the better the resolution, usually the smaller the volume under investigation. Several different approaches to overcome this challenge were presented at the Microscopy Conference in Vienna in 2021. These tools include array tomography, batch tomography, or scanning transmission electron tomography, all of which can nowadays be extended toward correlative light and electron tomography, with greatly increased 3D information. Here, we review these tools, describe the underlying procedures, and discuss their advantages and limits.
- Find related publications in this database (Keywords)
- Electron tomography
- Large volumes
- Structural cell biology
- 3D reconstruction
- Correlative light and electron microscopy
- Array tomography