Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Gaisl, T; Bregy, L; Stebler, N; Gaugg, MT; Bruderer, T; García-Gómez, D; Moeller, A; Singer, F; Schwarz, EI; Benden, C; M-L, Sinues, P; Zenobi, R; Kohler, M.
Real-time exhaled breath analysis in patients with cystic fibrosis and controls.
J Breath Res. 2018; 12(3):036013
Doi: 10.1088/1752-7163/aab7fd
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Singer Florian
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- Abstract:
- We aimed at defining profiles of volatile organic compounds in exhaled breath from patients with cystic fibrosis (CF) using a novel real-time mass spectrometry technique. In this prospective matched case-control study, 30 patients with CF, and 30 healthy control subjects were matched one-to-one according to age, gender, and smoking state. We performed exhaled breath analysis by untargeted secondary electrospray ionization-high resolution mass spectrometry (SESI-HRMS). Patients with CF (mean age 26.0 ± 13.0 years) and controls (mean age 27.9 ± 14.0 years) were analyzed using SESI-HRMS. 49 exhaled breath features were found to be altered (p-value < 0.05/q-value < 0.1) in CF patients, in comparison to healthy controls. The two most discriminating features showed a prediction AUROC of 77.1% (95% CI 62.2%-87.8%) with a specificity of 80.0% and a sensitivity of 63.3%. Levels of oxidative stress metabolites such as fatty acids were found to differ significantly between patients with CF and healthy controls. Furthermore, in patients with CF, 11 features correlated with the mucus concentration of Stenotrophomonas maltophilia bacteria. Exhaled breath analysis with SESI-HRMS allows the identification of CF specific compounds in real-time and may trace bacterial strains in affected patients with CF.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent - administration & dosage
- Adult - administration & dosage
- Breath Tests - methods
- Case-Control Studies - administration & dosage
- Child - administration & dosage
- Computer Systems - administration & dosage
- Cystic Fibrosis - diagnosis, microbiology
- Exhalation - administration & dosage
- Female - administration & dosage
- Humans - administration & dosage
- Lung - microbiology, pathology
- Male - administration & dosage
- Middle Aged - administration & dosage
- Prospective Studies - administration & dosage
- ROC Curve - administration & dosage
- Young Adult - administration & dosage
- Find related publications in this database (Keywords)
- SESI-HRMS
- mass spectrometry
- cystic fibrosis
- real-time analysis