Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Reitermaier, R; Ayub, T; Staller, J; Kienzl, P; Fortelny, N; Vieyra-Garcia, PA; Worda, C; Fiala, C; Staud, C; Eppel, W; Scharrer, A; Krausgruber, T; Elbe-Burger, A.
The molecular and phenotypic makeup of fetal human skin T lymphocytes
DEVELOPMENT. 2022; 149(8): dev199781
Doi: 10.1242/dev.199781
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- Co-authors Med Uni Graz
- Vieyra Garcia Pablo Augusto
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- Abstract:
- The adult human skin contains a vast number of T cells that are essential for skin homeostasis and pathogen defense. T cells are first observed in the skin at the early stages of gestation; however, our understanding of their contribution to early immunity has been limited by their low abundance and lack of comprehensive methodologies for their assessment. Here, we describe a new workflow for isolating and expanding significant amounts of T cells from fetal human skin. Using multiparametric flow cytometry and in situ immunofluorescence, we found a large population with a naive phenotype and small populations with a memory and regulatory phenotype. Their molecular state was characterized using single-cell transcriptomics and TCR repertoire profiling. Importantly, culture of total fetal skin biopsies facilitated T cell expansion without a substantial impact on their phenotype, a major prerequisite for subsequent functional assays. Collectively, our experimental approaches and data advance the understanding of fetal skin immunity and potential use in future therapeutic interventions.
- Find related publications in this database (Keywords)
- T cells
- TCR
- Skin
- Human
- Fetal
- Single-cell RNA-sequencing
- Flow cytometry
- Confocal microscopy