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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Meinitzer, A; Enko, D; Zelzer, S; Prüller, F; Alonso, N; Fritz-Petrin, E; Herrmann, M.
Development of a liquid chromatography mass spectrometry method for the determination of vitamin K1, menaquinone-4, menaquinone-7 and vitamin K1-2,3 epoxide in serum of individuals without vitamin K supplements.
Clin Chem Lab Med. 2022; 60(7):1011-1019
Doi: 10.1515/cclm-2022-0192
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Meinitzer Andreas
- Co-authors Med Uni Graz
- Alonso Lopez Nerea
- Enko Dietmar
- Fritz-Petrin Eva Verena
- Herrmann Markus
- Prüller Florian
- Zelzer Sieglinde
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- Abstract:
- OBJECTIVES: Vitamin K and metabolites have a beneficial role in blood coagulation, bone metabolism and growth. However, the determination of vitamin K concentrations in the blood in patients consuming a diet with naturally occurring vitamin K is currently challenging. We aim to develop a cost-effective and rapid method to measure vitamin K metabolites with potential application for clinics and research. METHODS: We developed a simple liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the determination of vitamin K1, menaquinone-4 (MK-4), menaquinone-7 (MK-7) and vitamin K1-2,3 epoxide in human serum and validated the method in a study cohort of 162 patients tested for carbohydrate malabsorption and in 20 patients with oral phenprocoumon intake. RESULTS: The overall precision (CVs) ranged between 4.8 and 17.7% in the specified working range (0.06-9.0 nmol/L for all analytes except for MK-7 with 0.04-6.16 nmol/L). In the malabsorption cohort samples, measured values were obtained for all different vitamin K metabolites except for vitamin K1-2,3 epoxide. This metabolite could be detected only in patients with phenprocoumon intake. The good performance of the method is especially achieved by the interaction of three factors: the use of lipase in the sample preparation, the use of an atypical fluorinated reversed phase column, and a logarithmic methanol gradient. CONCLUSIONS: The described method is able to determine the concentration of four vitamin K metabolites in a time-efficient, simple and cost-effective manner. It can be suitable for both routine clinics and research.
- Find related publications in this database (using NLM MeSH Indexing)
- Chromatography, High Pressure Liquid - methods
- Chromatography, Liquid - methods
- Epoxy Compounds - administration & dosage
- Humans - administration & dosage
- Phenprocoumon - administration & dosage
- Tandem Mass Spectrometry - methods
- Vitamin K - administration & dosage
- Vitamin K 1 - administration & dosage
- Vitamin K 2 - analogs & derivatives
- Find related publications in this database (Keywords)
- mass spectrometry
- menaquinon-4
- menaquinon-7
- vitamin K1
- vitamin K1-2
- 3 epoxide