Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schmidt, T; Jakesova, M; Derek, V; Kornmueller, K; Tiapko, O; Bischof, H; Burgstaller, S; Waldherr, L; Nowakowska, M; Baumgartner, C; Ucal, M; Leitinger, G; Scheruebel, S; Patz, S; Malli, R; Glowacki, ED; Rienmuller, T; Schindl, R.
Light Stimulation of Neurons on Organic Photocapacitors Induces Action Potentials with Millisecond Precision
ADV MATER TECHNOL-US. 2022; 2101159
Doi: 10.1002/admt.202101159
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- Führende Autor*innen der Med Uni Graz
- Schindl Rainer
- Schmidt Tony
- Co-Autor*innen der Med Uni Graz
- Bischof Helmut
- Burgstaller Sandra
- Kornmüller Karin
- Leitinger Gerd
- Malli Roland
- Nowakowska-Desplantes Marta
- Patz Silke
- Scherübel-Posch Susanne
- Tiapko Oleksandra
- Ücal Muammer
- Waldherr Linda
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- Abstract:
- Nongenetic optical control of neurons is a powerful technique to study and manipulate the function of the nervous system. This research has benchmarked the performance of organic electrolytic photocapacitor (OEPC) optoelectronic stimulators at the level of single mammalian cells: human embryonic kidney (HEK) cells with heterologously expressed voltage-gated K+ channels and hippocampal primary neurons. OEPCs act as extracellular stimulation electrodes driven by deep red light. The electrophysiological recordings show that millisecond light stimulation of OEPC shifts conductance-voltage plots of voltage-gated K+ channels by ≈30 mV. Models are described both for understanding the experimental findings at the level of K+ channel kinetics in HEK cells, as well as elucidating interpretation of membrane electrophysiology obtained during stimulation with an electrically floating extracellular photoelectrode. A time-dependent increase in voltage-gated channel conductivity in response to OEPC stimulation is demonstrated. These findings are then carried on to cultured primary hippocampal neurons. It is found that millisecond time-scale optical stimuli trigger repetitive action potentials in these neurons. The findings demonstrate that OEPC devices enable the manipulation of neuronal signaling activities with millisecond precision. OEPCs can therefore be integrated into novel in vitro electrophysiology protocols, and the findings can inspire in vivo applications.
- Find related publications in this database (Keywords)
- bioelectronics
- light stimulation
- neuronal excitation
- OEPC device
- photocapacitor
- voltage-gated ion channels