Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Werner, ER; Werner-Felmayer, G; Fuchs, D; Hausen, A; Reibnegger, G; Wachter, H.
Influence of interferon-gamma and extracellular tryptophan on indoleamine 2,3-dioxygenase activity in T24 cells as determined by a non-radiometric assay.
Biochem J. 1988; 256(2):537-541
Doi: 10.1042/bj2560537
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- Co-Autor*innen der Med Uni Graz
- Reibnegger Gilbert
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- Abstract:
- The indoleamine 2,3-dioxygenase (EC 1.13.11.17) activity in human T24 cells has been investigated in cell extracts by using a non-radioactive assay. It is enhanced in a dose-dependent manner up to 25-fold by interferon-gamma. The maximum reaction velocity is increased rather than the Km, which remains at 4 mumol/l. Induction of activity starts 3 h after stimulation and reaches a plateau at 21-48 h. Decreased stimulation was observed in the presence of high L-tryptophan concentrations.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line -
- Dose-Response Relationship, Immunologic -
- Enzyme Induction - drug effects
- Humans - drug effects
- Indoleamine-Pyrrole 2,3,-Dioxygenase - drug effects
- Interferon-gamma, Recombinant - pharmacology
- Isomerism - pharmacology
- Oxygenases - biosynthesis
- Time Factors - biosynthesis
- Tryptophan - pharmacology
- Tryptophan Oxygenase - pharmacology
- Urinary Bladder - enzymology