Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gualtieri, F; Nowakowska, M; von, Rüden, EL; Seiffert, I; Potschka, H.
Epileptogenesis-Associated Alterations of Heat Shock Protein 70 in a Rat Post-Status Epilepticus Model.
Neuroscience. 2019; 415: 44-58.
Doi: 10.1016/j.neuroscience.2019.06.031
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Nowakowska-Desplantes Marta
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- Abstract:
- Temporal lobe epilepsy is triggered by an initial insult, such as status epilepticus, that initiates the process of epilepsy development. Heat shock protein 70 (Hsp70) is a ubiquitously expressed molecular chaperone, involved in the inflammatory response that is upregulated after status epilepticus. Hsp70 has been described as an endogenous intracellular ligand of Toll-like receptor 4. It is released from damaged or necrotic tissue and by activated immune cells after an inflammatory event. So far, the time course and the pattern of epileptogenesis-associated alterations in Hsp70 expression have not been described in detail. Thus, we investigated immunohistochemical expression of Hsp70 in hippocampus, parahippocampal cortex, parietal cortex, amygdala, and thalamus following status epilepticus in a rat model of temporal lobe epilepsy. The impact of status epilepticus on Hsp70 expression varied during different phases of epileptogenesis, displaying a stronger effect in the early post-insult phase, a milder and more localized effect in the latency phase and no relevant effect in the chronic phase. Cellular-level characterization revealed that Hsp70 colocalized with the neuronal marker NeuN and with Toll-like receptor 4. No colocalization with the astrocytic marker GFAP or the microglia marker Iba1 was found. The intense neuronal Hsp70 upregulation during the early post-insult phase might contribute to the onset of excessive inflammation triggering molecular and cellular reorganization and generation of a hyperexcitable epileptic network. Therefore, development of multi-targeting strategies aiming at prevention of epileptogenesis should consider Hsp70 modulation in the early days following an epileptogenic insult.
- Find related publications in this database (using NLM MeSH Indexing)
- Amygdala - metabolism
- Animals - administration & dosage
- Astrocytes - metabolism
- Epilepsy, Temporal Lobe - metabolism
- Female - administration & dosage
- HSP70 Heat-Shock Proteins - metabolism
- Hippocampus - metabolism
- Inflammation - metabolism
- Microglia - metabolism
- Neurons - metabolism
- Parahippocampal Gyrus - metabolism
- Parietal Lobe - metabolism
- Rats - administration & dosage
- Rats, Sprague-Dawley - administration & dosage
- Status Epilepticus - metabolism
- Thalamus - metabolism
- Toll-Like Receptor 4 - metabolism
- Find related publications in this database (Keywords)
- epilepsy
- danger associated molecular patterns
- inflammation
- TLR4
- microglia
- nuclear factor-kappa B