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SHR Neuro Cancer Cardio Lipid Metab Microb

Künnapuu, J; Tauscher, PM; Tiusanen, N; Nguyen, M; Löytynoja, A; Arora, K; Shimmi, O.
Cleavage of the Drosophila screw prodomain is critical for a dynamic BMP morphogen gradient in embryogenesis.
Dev Biol. 2014; 389(2): 149-59. Doi: 10.1016/j.ydbio.2014.02.007
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Co-authors Med Uni Graz: Tauscher Petra
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Abstract:: Dorsoventral patterning of the Drosophila embryo is regulated by graded distribution of bone morphogenetic proteins (BMPs) composed of two ligands, decapentaplegic (Dpp) a BMP2/4 ortholog and screw (Scw) a BMP5/6/7/8 family member. scw(E1) encodes an unusual allele that was isolated as a dominant enhancer of partial loss-of-function mutations in dpp. However, the molecular mechanisms that underlie this genetic interaction remain to be addressed. Here we show that scw(E1) contains a mutation at the furin cleavage site within the prodomain that is crucial for ligand production. Furthermore, our data show that Scw(E1) preferentially forms heterodimers with Dpp rather than homotypic dimers, providing a possible explanation for the dominant negative phenotype of scw(E1) alleles. The unprocessed prodomain of Scw(E1) remains in a complex with the Dpp:Scw heterodimer, and thus could interfere with interaction of the ligand with the extracellular matrix, or the kinetics of processing/secretion of the ligand in vivo. These data reveal novel mechanisms by which post-translational regulation of Scw can modulate Dpp signaling activity.
Find related publications in this database (using NLM MeSH Indexing): Amino Acid Sequence - administration & dosage; Animals - administration & dosage; Blastoderm - embryology, metabolism; Bone Morphogenetic Proteins - metabolism; Drosophila Proteins - chemistry, metabolism; Drosophila melanogaster - embryology, metabolism; Embryo, Nonmammalian - metabolism; Embryonic Development - administration & dosage; Ligands - administration & dosage; Molecular Sequence Data - administration & dosage; Mutant Proteins - chemistry, metabolism; Mutation - genetics; Protein Multimerization - administration & dosage; Protein Structure, Tertiary - administration & dosage; Signal Transduction - administration & dosage; Transforming Growth Factor beta - chemistry, metabolism
Find related publications in this database (Keywords): Bone morphogenetic protein; Proprotein convertase; Dorsal-ventral patterning; Post-translational regulation; Decapentaplegic (dpp)