Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Najdekr, L; Blanco, GR; Dunn, WB.
Collection of Untargeted Metabolomic Data for Mammalian Urine Applying HILIC and Reversed Phase Ultra Performance Liquid Chromatography Methods Coupled to a Q Exactive Mass Spectrometer.
Methods Mol Biol. 2019; 1996: 1-15.
Doi: 10.1007/978-1-4939-9488-5_1
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- Co-Autor*innen der Med Uni Graz
- Rodriguez Blanco Giovanny
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- Abstract:
- Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) is the most frequently applied analytical platform in the untargeted metabolomic study of mammalian urine. Here we describe two complementary UPLC-MS methods for metabolomic analysis or urine, a reversed phase C18 method and a hydrophilic interaction liquid chromatography (HILIC) method. We discuss the inclusion of pooled quality control (QC) samples and a recommended analysis list construction. Up to 96 injections can be performed every 24 h, and up to 2000 metabolites can be routinely detected.
- Find related publications in this database (using NLM MeSH Indexing)
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- Humans - administration & dosage
- Metabolomics - instrumentation, methods
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- Urine - chemistry