Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Smolle, J; Helige, C; Tritthart, HA.
An image analysis and statistical evaluation program for the assessment of tumour cell invasion in vitro.
Anal Cell Pathol. 1992; 4(1):49-57
Web of Science
PubMed
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Smolle Josef
- Co-Autor*innen der Med Uni Graz
- Helige Christine
- Tritthart Helmut
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- Abstract:
- Tumour cell invasion is a complex process, which is essential for the formation of metastasis and is therefore of critical clinical importance. For detailed investigations of the invasive process, quantifiable in vitro models of invasion are necessary. In this study we describe an image analysis procedure and a statistical program which facilitate an objective analysis of experiments carried out using the embryonic chick heart invasion model of Mareel. Tumour multicellular spheroids are confronted with embryonic chick heart fragments in culture and are sampled after different time intervals for up to 7 days. Immunohistological sections are then evaluated by an image analysis procedure which provides 9 parameters indicating invasion, proliferation and destruction taking place in the confrontation cultures. The data obtained by image analysis are further evaluated by a statistical program which describes the change with time of each parameter by means of linear regression analysis. Thus the data obtained at various time intervals serve as the source data for a single statistic, namely the slope of the regression line. Confidence intervals and statistical differences between various experiments can be calculated. In order to make the procedure more comprehensible in biological terms, the program provides a full text interpretation of the experimental results. The image analysis procedure in conjunction with statistical evaluation and text interpretation provides a comprehensive tool for the quantitative assessment of experimental invasion in vitro.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Chick Embryo -
- Heart - embryology
- Image Processing, Computer-Assisted - embryology
- Immunoenzyme Techniques - embryology
- Melanoma, Experimental - pathology
- Neoplasm Invasiveness - pathology
- Regression Analysis - pathology
- Tumor Cells, Cultured - pathology
- Find related publications in this database (Keywords)
- Invasion
- Confrontation Culture
- Embryonic Chick Heart
- Image Analysis
- Regression Analysis
- Interpretation